Journal: Blood Advances
Article Title: Rh alloimmunization in chronically transfused patients with thalassemia receiving RhD, C, E, and K matched transfusions
doi: 10.1182/bloodadvances.2020003732
Figure Lengend Snippet: Alloimmunization and genotype-predicted Rh antigen expression among chronically transfused patients with SCD receiving prophylactic C, E, and K matched red cells by simple transfusion. (A) Antibody specificities detected among 48 chronically transfused patients with SCD. Columns for each specificity indicate patients’ corresponding antigen status (positive or negative) as reported by standard serologic or genotyping methods. (B) RHD and RHCE genotype-predicted Rh antigen expression among patients with SCD. Partial antigens predicted from genotypes with variant alleles that result in Rh epitope(s) missing and absence of conventional antigen. RHD*DAU0 or RHCE*ce48C has not been shown to encode Rh proteins lacking epitopes and is considered altered antigens.
Article Snippet: RH genotyping was performed with RHD and RHCE BeadChip arrays (Bioarray/Immucor), and polymerase chain reaction–based assays, as described previously.
Techniques: Expressing, Variant Assay